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The dataset used, which comprises 515 alleles found in more than 831 isolates spanning a great taxonomical host range, reflects a substantial sampling diversity of WSP and allowed grouping of recently diverged WSP proteins and HVR motifs.
Here we focus on averages across participants in the 12 states, which reduces the substantial sampling error and averages across idiosyncratic state differences.
It appears that until substantial sampling of lower taxa among flatworms is performed, and more studies on stem cells in non-rhabditophoran flatworms are available, none of the competing phylogenetic hypotheses can be favored.
This is particularly relevant to identify selection signatures based on pooled sequencing data, for which estimates of features, such as F ST, contain substantial sampling error.
Not surprisingly for such small samples, unpenalized estimates were subject to substantial sampling variation and were most variable for pairs of traits with lowest h.
The rarefaction curve also suggests we have underestimated diversity, indicating a more reasonable estimate might require substantial sampling (up to 30 40 clones per individual).
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A substantial sample of upper and lower canines (and lower third premolars) showed that the upper canines of Ardipithecus did not sharpen (hone) as did those of all fossil and modern apes.
This requires substantial sample handling, quality assurance, and data reduction.
This enabled realization of highly parallel and automated fluidic processes with substantial sample economy advantage.
Including those we scored at zero, the mean score is 36.1 with a standard deviation (SD) of 22.3, indicating substantial sample variation (Table 1).
Although this enabled access to a more diverse and substantial sample that could otherwise be reached, it also raises issues about the integrity of the data.
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