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We advanced a working hypothesis that the storage of WB samples in glass tubes would result in a more rapid and substantial pH drop and inactivation of several enzymes involved in coagulation.
At external pH 6, the initial cytoplasmic pH was significantly lower than the cytoplasmic pH maintained at external pH 7 (see Fig. 3A), but the cells still maintained substantial pH homeostasis, with a full unit of ΔpH (Fig. 3D).
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Heat shock has been found to lead to a substantial decrease (~1 pH unit) in cytoplasmic pH (Ayer et al. 2013) which perturbs redox regulation in the cytoplasm and which would stress the cellular capacity to maintain pH homeostasis in the absence of the vacuolar V-ATPase.
However, although the binding strength was lower, both media exhibited substantial selectivity at pH 7.5.
Within our treatment conditions, the proportion of HF species becomes substantial only below pH 5.0 (Figure 2).
Reaction (2) suggests that, except in the special case where x = 1 + y, substantial shifts in pH should be observed as oxidation occurs, unless the solution is buffered.
PH: substantial contribution to the conception and design of the study, data analysis and interpretation; involved in drafting and revising the manuscript and approves final version JB: contribution to the study design and substantial contribution to data collection, analysis and interpretation.
For example, an alkali loading of 0.10 g NaOH per g biomass is an alkali concentration of only 5 g/L at 5% (w/v) biomass solids concentration and 20 g/L at 20% (w/v) solids, resulting in substantial differences in pH.
Substantial overlap of Ph-responsive genes was identified in super-susceptible (L94) and partially-resistant (Vada) lines.
We have observed a substantial decrease in growth media pH from pH 7.5 down to pH 2.5 during the log phase, which was more evident and faster with H. werneckii growing in 4.5 M NaCl than in 3 M NaCl (unpublished data).
At all pH's, substantial coverages are observed only at potentials in the low cd region.
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