Sentence examples for substantial off from inspiring English sources

New York theatergoers may know Mr. Letts as the author of the trailer-trash gore-fest "Killer Joe" and the equally sanguinary psychological thriller "Bug," both of which had substantial Off Broadway runs.

And if the serfs seek redress the High Court will slap them down before they can get anything substantial off the ground.

However, if DMD protein expression need only be 29% of normal levels to alleviate symptoms of muscle weakness [18], a dosing regimen for HDAC inhibitors may be possible that induces specific exon skipping without substantial off target effects and toxicity seen at high doses in this and other studies [37].

Both teams went through fairly substantial off-season makeovers.

In its latest report on Angola, the fund criticized the government for repeatedly making substantial off-budget expenditures and its failure to track state revenue.

It has been estimated from studying the genomes of humans, C. elegans and S. pombe that about 10% of possible siRNAs have substantial off-target effects.

But occasionally they are found to show substantial off-target binding that accounts mainly for "drug cytotoxicity".

Another commonly used Cl− channel blocker, DIDS, substantially reduced P-selectin expression and almost abolished αIIb β3 activation, suggesting that it may have substantial off-target effects, and so was not used further in this study.

However, previous studies reported substantial off-target activities of CRISPR system in human cells, and the enormous putative off-target sites are labor-intensive to be validated experimentally, thus motivating bioinformatics methods for rational design of CRISPR system and prediction of its potential off-target effects.

Early studies have shown that Cas9-mediated cleavage can be abolished by single mismatches at the sgRNA DNA target site interface [ 7, 8]; however, more systematic investigations revealed that the CRISPR system could cause substantial off-target effects in human cells which resulted from the binding between sgRNA and imperfectly complementary DNA sequences in the genome [ 12– 12].

However, in contrast to evidence for substantial off-target site editing in cell-based systems (Fu et al. 2013), emerging data indicate that CRISPR-induced editing events are highly specific in mouse embryos (Wang et al. 2013; Yang et al. 2013a).