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Substantial gel dilution (80%:diluent/gel, v/v) was required before diffusion coefficients rose to even two orders of magnitude lower than those in water.
They reported substantial gel stability on storage, though chemical stability remained undisclosed [ 86].
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On the other side, Nup153 localizes far to the nuclear side and also contributes a substantial share of gel-forming FG domain mass.
If the patient has substantial laxity, a cohesive gel implant with less volume should be fitted.
We demonstrate room temperature (20 °C) self-healing, and substantial recovery (68 96%) of gel rigidity of gelatin, a polypeptide, ionogels (made in 1-ethyl-3-methylimidazolium chloride ionic liquid (IL) solutions via thermal treatment, IL ≤ 5% (w/v)) after they were cut using a surgical blade.
Wild-type and mutant dissociation constants (Kd values), determined using gel shifts, displayed no substantial differences, and were of the same order as kM (2 20 nM).
Analysis of the gels by densitometry revealed substantial changes in protein expression in some of the protein spots we analyzed.
In fact, there are substantial amounts of dimers in their gels.
The substantial increase in storage modulus of the gel filled systems can be attributed to the presence of three-dimensional networks of crosslinked gel which provide greater resistance to dynamic deformation.
The additional analysis and testing via mutants, gels, FRET and SPR provide substantial added impact.
Gel permeation chromatographic analyses showed that substantial quantities of high molar mass material had been formed.
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