We have found that large numbers of differentially expressed genes, with substantial expression changes, are missed or misidentified when data are normalized with methods that assume lack-of-variation.
In the synovial membrane of patients with RA, there is a substantial expression of IL-15, predominantly expressed in macrophages but also in fibroblast-like synoviocytes and endothelial cells [ 3- 5].
However, based on immunofluorescence results we do not observe substantially different levels of over-expression of each isoform, nor is there substantial expression, if any, outside of the normal fru P1 expression pattern (see Additional file 8: Figure S2).
This document promises to be Mayor Michael R. Bloomberg's first substantial expression of city poverty policy, however cautiously it may be drafted to avoid political minefields.
It was based on the notion of developing the intellect for substantial expression and it helped to fuel "big thinking" – the food of innovation.
Real time PCR analysis confirmed the observation that both BMP4 and Wnt3a were able to induce substantial expression of BMP4, Wnt3 and nodal.
In the cerebral cortex of E16 rats, substantial expression of both APP-BP1 and APP mRNAs was observed in the ventricular zone.
Staining for intracellular IFNγ showed that iNKT cells already had substantial expression of IFNγ at 6 h, and this continued to increase at 24 h (not shown).
Substantial expression of EGFP was also detected in the PECS and retina following intravitreal injection, albeit at levels multi-fold lower than that observed in the lens.
The importance of other Group IV SERA genes in this lineage cannot be ruled out because of substantial expression of these genes at the blood stage [7].
Interestingly, Jurkat E6.1 T cells and APBTs also had substantial expression of the costimulatory receptors CD28 and CD2, whereas HuT78 T cells had no detectable expression of these molecules (Table 2).
