Exact(3)
Unreacted substances were washed off 4 5 times by repeated washing procedure in which dried product at each stage was dissolved in water, precipitated with excess ethanol, centrifuged at 7000 rpm and supernatant discarded.
Unbound substances were washed away, and an enzyme-linked polyclonal antibody specific for mouse CHI3L1 was added, followed by a wash to remove unbound antibody enzyme reagents.
After unbound substances were washed away, biotin conjugated to a secondary antibody raised against CPT1B was added, and the plate incubated for 1 h at 37 °C.
Similar(57)
This reaction requires days to develop, but it continues to work after the substance is washed off.
The resultant polymeric substance was washed with acetonitrile/methanol mixture in order to remove AOT and the residual reagents.
The black substance was washed using a mixture of ethanol and toluene, and vacuum dried in an oven at 60°C overnight.
The deaths follow those of scores of other birds covered in a "sticky substance" being washed up on the south coasts of the counties in the past week.
The substance is washed, heated, then flaked.
Repeated rinse of ethanol, PBS and DI water only blueshifted the wavelength 3 nm, because some unspecific bonded substances on the sample were washed away.
After recording of this first contraction, vessels were washed and pharmacological substances were added both to the bath and to the perfusion solution inside the vessel for one hour.
Drugs were washed out before the next substance was added.
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