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Substances were separated on a fused silica capillary column (HP-Innowax, 60 m × 0.25 mm I.D., film thickness 0.25 μm).
The precipitated HA-like substances were separated by centrifugation from the soluble fulvic acid that remained in the supernatant.
Also, other important substances were separated using an SLM system with the aim of using supported liquid membranes in bioindustrial processes.
Substances were separated on an endcapped, 5-µm, LiChroCART® 125-4 RP18 column (Merck, Darmstadt, Germany) run at a flow rate of 1 ml/min.
Root exudates and test substances were separated under identical conditions.
The neutral and phenolic substances were separated with potassium hydroxide (0.5 M in 50% EtOH) and hexane partitioning.
Similar(54)
Substances are separated by this method on the basis of their different solubilities in two immiscible liquids.
Due to the fact that standard substances of branched PFHpS and PFHxS were not available, it was not possible to ascertain that all the branched substances are separated completely from the linear ones.
Interferences of PFOS with taurodeoxycholic acid are excluded, because both substances are separated chromatographically and furthermore the relation of the two most intensive transitions of PFOS in comparison to a standard solution was used to check possible interferences.
Substances are separated based on their electrophoretic mobility, which is proportional to their charge to size in the interior of a small capillary filled with an electrolyte.
Graphitic substances in the quinoline-insoluble fraction were separated through precipitation in acetone.
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