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For quantification of products formed commercially available standard substances were measured with concentrations from 0.5-10 0.5-10 the creation of calibration curves.
Concentrations of the substances were measured in chicken eggs, beef and poultry liver, pork, game and fish offal, poultry and game meat, salt water and fresh water fish, French fries, honey, and drinking water.
Induction by Fe3+/ascorbate system: the reaction mixture containing rat liver and kidney homogenate (0.1 ml, 50% w/v) in Tris HCl (30 mM), ferrous ammonium sulfate (0.16 mM), ascorbic acid (0.06 mM), and the reaction mixture was incubated for 1 h at 37°C, and the resulting thiobarbituric reacting substances were measured (Samy et al. 2006).
All citied substances were measured by automated known spectrophotometry and colorimetric analysis.
The dioxins and related substances were measured only in paired serum and milk samples.
Using TBA, TBARS (thiobarbituric acid reactive substances) were measured in the tissue homogenate [ 23].
Similar(49)
The level of TBARS (thiobarbituric acid-reactive substances) was measured according to the published protocol [ 18].
Lipid peroxidation as a measure of thiobarbituric acid reactive substances was measured following the standard procedure [ 41].
The pink colour representative of thiobarbituric acid reactive substances was measured at 532 nm and protein concentration determined.
The concentration of TBA reactive substances was measured at 532 nm using a standard curve of MDA, and the results were expressed as nmol MDA/mg protein.
The concentrations of the test substance were measured using HPLC with photometric detection.
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