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The active substances were analysed in the runoff samples and the eluates from the laboratory tests by ultra-high-performance liquid chromatography coupled with UV detection at BAM, and in runoff samples by liquid chromatography coupled with tandem mass spectrometry at MPA Eberswalde.
In the present study a number of predetermined biochemical substances were analysed.
Furthermore, databases and lists of active substances were analysed, such as the register of detergents and cleaning agents of the German Federal Environmental Agency, which provided (product-independent) summary analyses, and the German drug directory "Rote Liste".
The toxicological data for the substances were analysed on the basis of reviews, supplemented by original literature, and summarised and evaluated with respect to the exposure data (for details cf. Hahn et al. [ 16]).
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The substance was analysed by the Maritime and Coastguard Agency.
The above findings are based on the assumption that the concentrations of substances that were analysed for, but not detected (NDs) were zero.
Quantitative outcomes (frequency of substance use) were analysed using a random effects model.
The substance P and CGRP quantifications were analysed with a 2-way ANOVA.
Only the three clones (FJ, FBM and FBP; for symbols see Methods) that contained stilbenes and emodin in higher concentrations were analysed for organic substances.
To allow for comparison with the results of Naim et al. (1997), those parts of the dendrites that lay within the plexus of substance P-immunoreactive axons (superficial dendrites) were analysed separately from parts of dendrites that lay ventral to this plexus (deep dendrites).
Also extracted drug substances from the samples of the perfused tissues were analysed.
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