The serum antiradical capacity of water-soluble (ACW) and the antiradical capacity of lipid-soluble (ACL) substances (ACW-Kit and ACL-Kit; Analytik Jena AG, Jena, Germany) were determined by photochemiluminescence detection using a Photochem analyser (Analytik Jena AG, Jena, Germany).
Malondialdehyde, an oxidative stress marker in sputum supernatants, was measured with a commercially available thiobarbituric acid reactive substances assay kit (Cayman) according to the manufacturer's instructions.
The level of oxidation was assessed using a thiobarbituric acid reactive substances assay kit (Cayman, Ann Arbor, MI) and estimated as 59.5 ± 0.5 nmol malondialdehyde per mg ApoB protein for oxLDL and non-detectable for nLDL.
Substance P and bradykinin were measured using commercial enzyme immunoassay kits (substance P kit from Cayman Chemical, Ann Arbor, MI, USA; and bradykinin kit from Bachem, Bubendorff, Switzerland).
Plasma levels of malondialdehyde were quantified using the thiobarbituric acid reactive substance assay kit (Oxitek-ZeptoMetrix CorporatioNY BUSAalo, NY, USA) following the manufacturer's protocol.
The concentration of total protein was measured using a protein assay reagent (Bio-Rad Laboratories, Inc).. Lipid peroxidation was estimated by measuring the concentration of MDA using a thiobarbituric acid-reactive substance assay kit (BioAssay Systems, CA, USA).
Thiobarbituric acid reactive substances (TBARS) assay kit was purchased from ZeptoMetrix Inc , USA
The levels of MDA equivalents were determined by thiobarbituric acid reactive substances (TBARS) assay kit (Cayman Chemical Company, Ann Arbor, Michigan, USA).
Lipid peroxidation was estimated by determining the MDA content using a thiobarbituric acid reactive substances (TBARS) assay kit (BioAssay Systems, CA, USA).
