This method can be widely applied to study some toxic substances' effects on Japanese medaka's respiratory rhythms and to assess the degree of risk of the water environment.
Here, we present the technical performance verification studies of the cobas EGFR Mutation Test, including studies of the analytic sensitivity, internal and external reproducibility, minimal tumor content, interfering substances, effects of necrosis, and cross-reactivity with other mutations.
Typically, a substance effect in the range of 1 to 10 % is set as tolerable level.
In this case, distinguishing a substance effect from the background mortality is more crucial, (KNK, SS, Wosniok W, submitted), [43].
However, from an environmental perspective, it is most important to safely identify the concentration of the substance effect which lies above the effect under control condition (spontaneous effect).
On the one hand, ecotoxicological data exist only for carbamazepine and phenytoin; on the other hand, according to [34], substances with similar targets can add their effects to each other and cause effects below the single substance effect concentrations.
As an example, if the substance effect increases and consequently the effect rate rises by 0.000001 (= 0.0001 %), the experimental group must contain at least 1/0.000001 = 1,000,000 objects to make the expected increase in response (by only one object) visible.
The two-way ANOVA reveals a Substance effect (F = 13.56, p<0.001) and a Group x Substance interaction (F = 13.09, p<0.001).
The two-way ANOVA indicates a Group effect (F = 7.81, p<0.01), but no Substance effect (F = 2.22, ns) and no Group x Substance interaction (F = 1.94, ns).
Arrow 2 shows the succeeding drop in evoked potential amplitudes, which were prevented in the NE group possibly due to a substance effect.
