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For the administration of the substance, we used a 22-G needle.
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Using the second validation set, the SDEP is still comparable with the experimental variability, which range from 0.75 to 0.42 for the sets of substances we used (see above).
In order to test the presence and the function of seminal fluid substances, we used the hermaphroditic pond snail Lymnaea stagnalis (L).
For the propolis extraction of poorly soluble in water and oil substances, we used not only extraction at 70 °C temperature, but also additional solvent PEG.
Since Chlorella, a unicellular green alga, contains various antioxidant substances, we used a mouse model of enhanced oxidative stress to investigate whether Chlorella could prevent muscle atrophy.
For the delivery of the tussigenic substances we used Omron MicroAir portable ultrasonic nebulizer and the patients were asked to take five consecutive tidal breaths of a given concentration.
This year, we found out that a lot of the substances we use to get off our faces partying on the weekends could actually help with real-life health issues.
To measures substance use we used the Alcohol Use Disorders Identification Test AUDITT) [ 23] and the Drug Use Disorders Identification Test DUDITT) [ 24].
For substance use, we used no cigarette smoking, no areca chewing and no alcohol consumption as baselines and compared these baselines to lifetime consumption.
As a negative substance control we used lactose, which induced no considerable cell damage (13% after an exposure time of 30 minutes).
In order to strengthen our hypothesis (i.e., negatively worded items have a wording effect on substance abusers), we used another statistical method (confirmatory factor analysis, CFA) to justify the results.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com