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The nanoparticle agglomerates substance was prepared in an isotonic solution using ultrasonic disperser (f, 35 kHz, N, 300 W, A, 10 mkA) in the following mode: thrice-repeated dispersion for 1 min each, recess for 3 min.
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Stock solutions of 100 mg/L of each substance were prepared in methanol and stored at 4°C in amber glass bottles that were previously washed with methanol.
Linearity test solutions for related substance were prepared by diluting the impurity stock solution (as described in the section of 'Analytical procedure') to the required concentrations.
For this procedure, once the substance is prepared, the consumer should be asked to put precisely the amount they consume in a sealable container [84].
A standard stock solution containing a mixture of saccharin, phenacetin, paracetamol, and methaqualone having a concentration of 2 mg/mL of each of these substances was prepared in the mobile phase.
Fat and oil processing, method by which animal and plant substances are prepared for eating by humans.
All the chemical reference substances, including clematichinenoside AR and related substances were prepared in our laboratory, and the purity of each compound was confirmed to be higher than 98% by HPLC.
All substances were prepared fresh daily and administered at a volume of 100 μL.
Secreted substances were prepared by culturing B. anthracis (delta Ames) in LB media overnight.
A series of working solutions for all the standard substances were prepared by serial dilutions with methanol.
Stock solutions of standard substances were prepared at the concentration of 1 mg/ml in methanol and stored at −20 °C.
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