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Evidently, also homogeneity of the test substance preparations influences NM uptake upon oral delivery.
Test substance preparations were produced daily and were kept homogenous until administration by continuous stirring with a magnetic stirrer.
Also BaSO4 was not distributed homogeneously in the test substance preparations, and only contained an average of 6.5 g BaSO4 per 100 g (Table 2).
The test substance preparations were administered daily by gavage over a period of 4 weeks to groups of 5 male and 5 female rats.
Whereas homogeneity of the test substance preparations was satisfactory in the surface-functionalized SiO2 and ZrO2 NMs, the non-surface-functionalized SiO2·naked and the powder BaSO4 were not distributed homogeneously.
For this purpose, three separate samples of the test substance preparations were taken from the bottom, middle, and top layers of the vials (which would necessarily have the same test substance concentrations in homogenous suspensions).
Similar(49)
Are nanomaterial test substance preparation and characterization sufficiently standardized to ensure reproducibility of test results in accordance with specified parameters?
Further investigations, however, are necessary to develop and assess standardized procedures for test substance preparation for oral administration ensuring reliable substance homogeneity.
Restricted chemicals are listed in Annex XVII of the REACH regulation (Annex XVII, Restrictions on the manufacture, placing on the market and use of certain dangerous substances, preparations and articles).
This law defines pharmaceutical drugs as substances or preparations from substances with which physical or mental suffering can be cured, lessened, prevented or recognized.
The aims of the proposed directive are to guarantee the free movement of chemical substances and preparations and to improve health protection and consumer safety by prohibiting the placing of CMR substances on the market for use by the general public.
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