Subsets of cells expressing each functional marker were analyzed by Boolean gating.
Subsets of cells and/or genes were picked for further analysis and reclustered accordingly using the same clustering method.
Many published studies refer to subsets of cells, e.g., CD11c (hi), as DCs and distinguish them from macrophages.
The ALCAM expression is limited to subsets of cells in most adult tissues.
One fixed and one random effect was considered, in order to analyze the difference between subsets of cells and to consider the replicates variance, respectively.
This is especially true if one identifies subsets of cells on a fluorescence-activated cell sorting profile that express high or low levels of a marker.
First, it is necessary to consider that the T-cell population is diverse and contains several subsets of cells that have distinct functions and characteristics.
"These subsets of cells may explain different healing potentials in different people," said senior author Valerie Horsley, associate professor of molecular, cellular, and developmental biology.
hESC cultures are known to be heterogeneous, but whether subsets of cells contribute differently to functional assays has yet to be examined.
These unique subsets of cells, represented by almost every immune cell type, function to control responses of effector immune cells.
In the context of the adaptive immune system, where the activation and expansion of subsets of cells with unique specificities is essential, epigenetic regulation of the fidelity of cell identity is paramount.
