A subset of amplified cDNA inserts (435 of 704) were checked by electrophoresis on a 1% agarose gel, for quality control and to estimate average insert size.
Variegated or mosaic expression of Venus may even be exploited to target expression of pro-apoptotic genes, recombinases or transforming oncogenes only to a subset of cells, e.g., by inserting an IRES-element in the 3'UTR of Venus, followed by the cDNA of interest.
Based on the analyses of a subset of clones, the average insert size was estimated at 90 kb, ranging from 50 to 130 kb.
A survey of the size of the insert in a subset of 48 clones, as assessed by resolving a polymerase chain reaction (PCR) product on 1 % agarose gels, revealed an average insert size of 750 bp.
Informally, we estimate the total number of matepairs likely to be mapped to the locus and compute the probability that some subset of these deviates from the mean insert size by at least as much as the observed data.
Yet, it would be possible to scale to larger sizes by using column-generation techniques, whereby only a small subset of the generated set of routes is initially inserted in the problem, and new ones are inserted as needed using standard duality arguments.
Initial quality control of each library included quantification of: the titer of each one, the frequency of recombinant/insert-less clones and the average insert size (using a subset of 20 recombinant clones).
The inserts were deleted from a subset of these lines by FLP recombinase.
Additional sequence was generated from a putative, nonredundant subset of 11,904 cDNA clones, which were pooled, their inserts fragmented,and sequenced by Illumina Genome Analyzer methods (SRA accession SRR2119182).
In a subset of animals, a chronic intravascular LAD catheter was inserted in place of the femoral artery infusion catheter.
