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Mice were treated with either sterile saline (vehicle), 1 µg Ang-1 or 4 µg Ang-1 per animal at initiation of HVT-ventilation and subsequently ventilated for 5 hours.
The mouse was subsequently ventilated with a Harvard pump ventilator (tidal volume = 0.4 ml, frequency = 120 breaths/min, positive end-expiratory pressure 2.5 3.0 cm H2O) and the tail vein was cannulated with a 27 gauge needle for injection of the methacholine challenge.
23 (22%) were subsequently ventilated.
STAI-Y1 was higher and uncertainty more frequent in subsequently ventilated patients, who had shorter onset-admission delay and greater weakness but not a lower VC.
A feeling that symptoms and weakness were progressing and a feeling of breathlessness and suffocation were greater in subsequently ventilated patients as was the dyspnoea VAS (mean difference 1.2, 95% CI 0.2 to 2.3, p=0.015).
Here, both LPS and ropivacaine led to a slight increase in MPO activity compared to control in mice that were subsequently ventilated with NTV (p = 0.02 for LPS-R-NTV, p = 0.027 for NS-R-NTV, Figure 2D).
Similar(53)
Eleven lambs with gestational age 119 141 days (term =145 days) delivered by cesarean section were given surfactant and subsequently mechanically ventilated.
Subsequently, they were artificially ventilated after surgical tracheotomy by a ventilator (Ventilator 710, Siemens-Elema, Sweden) followed by a 0.2 mg/kg/h pancuronium bromide i.v. injection.
VC, one of the most powerful predictors, was not significantly different between patients who did or did not subsequently require MV. 3 9 23 Moreover, additional risk factors such as inability to lift the head, facial palsy and bulbar dysfunction were not more frequently present in patients who subsequently were mechanically ventilated.
A review of preterm infants >28 weeks' GA receiving NCPAP as their initial mode of respiratory support at the participating Australian centres (unpublished data) showed that 17% of such infants were subsequently intubated and ventilated, within 72 h of starting treatment.
The animal was subsequently intubated and mechanically ventilated.
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