An illustration involving the Wellcome Trust Case-Control Consortium data shows several genome-wide-significant associations, subsequently validated, that were not significant in the standard analysis.
Using an affinity chromatography approach, we identified and subsequently validated that ICG-001 binds specifically and with high affinity (approximately 1 nM) to the coactivator CBP, but, importantly, not to its closely related homolog p300, despite the fact that these two coactivators are up to 93% identical, with even higher homology, at the amino acid level.
And what happened later validated that.
Subsequently, we validated that miR-141 directly recognized the 3′-UTR of the HNF-3β transcript and downregulated HNF-3β expression.
In essence, whether you're in a car or on foot an officer will believe -- and a court will subsequently validate the belief -- that you can be detained indefinitely until the officer decides that he's done with you.
Of the seven studies included in the meta-analysis, two prospective cohort studies identified cases by linkage with national health registers [ 7, 12], while one prospective cohort used self-reported RA cases that were subsequently validated [ 11], a method that could have led to under-detection of cases who did not self-report RA.
One of the most successful discoveries in this area, made by our group and others, and subsequently validated worldwide, is that hypermethylation of the DNA repair enzyme MGMT is associated with a good response to nitrosurea alkylating agents in glioma.
Another high-scoring candidate module ("55-68") was subsequently validated in prospective experiments that confirmed a predicted protein-protein interaction.
Subsequently, we experimentally validated that the candidate cofactors Phd1, Cin5, Yap6, and Skn7 by showing that they physically interact with Tup1 and/or Ssn6.
The reference dataset was used as the hypothesis generating dataset, and genes with a FDR adjusted p-value < 0.1 were included in the gene set that was subsequently validated using the independent validation dataset.
