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Subsequently, titrated volume of 1 % chitosan solution was added to the PLGA solution under constant stirring at room temperature.
Next, hyperoncotic albumin (20%) solution was administered by 200-ml boluses over 60 min twice on the first day and subsequently titrated toward a serum albumin level of 30 g/L.
Positive samples were subsequently titrated using serial 2-fold dilutions.
Serum was obtained by spinning whole blood at 4500 r.c.f. for 15 min, and subsequently titrated by plaque assay in duplicate on Vero cells.
Viral stocks of RSV Long strain (originally isolated in Baltimore, MD, 1956) and RSV B1 (ATCC VR-1580) were prepared in HEp-2 cells and subsequently titrated to determine the optimal infectious dose (1 3 pfu/cell) for use in the micro neutralization assay.
These blood samples were subsequently titrated to determine the level of WNV viremia.
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Therefore, current guidelines recommend using an initial FiO2 of 0.21 0.30 and to subsequently titrate FiO2 according to the infant's SpO2 measured by pulse oximetry in order to avoid hyperoxia [48 50].
Dosages slightly increased from Visit 1 to Visit 3, indicating that the physician prescribed Equasym XL® according to recommended clinical practice, i.e. starting with a lower dose and subsequently titrating up according to the needs of the patient.
In addition, Aβ43 was shown to deposit earlier than other Aβ species in the brain of mouse models of AD [ 38] and to be surrounded by other Aβ species in brains of AD patients [ 29], further suggesting its ability to nucleate and subsequently titrate other Aβ species.
Supernatants were harvested every 6 h for the first 36 h of infection and every 12 h subsequently, then titrated by plaque assay as previously described [22].
In hypotensive patients with ALF, intravascular volume should be repleted first with normal saline, and vasopressors should be administered subsequently to titrate the MAP to >75 mmHg and CPP to 60-80 mmHg.
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