Even subsequently, they used this framing to make laws more equitable.
Subsequently, they used direct sequencing, restriction fragment length polymorphism analyses (RFLPs), and a real-time PCR-based allelic discrimination assay to genotype FOXL2 in a large collection of tumors.
Subsequently they can use the simulation environment based on the link and access provided by the administrators.
After 12 h of incubation, the enzymes were recovered using centrifugation 15 min at 5, 000 g. Subsequently, they were used for a second hydrolysis cycle using the same conditions described above without adding enzymes.
Subsequently, they were used to establish optimal enzyme assay conditions.
Subsequently, they were used interchangeably because they had identical properties with two key components (GR and the reporter gene, GREtkLUC) in the mechanistically sensitive competition assay.
Subsequently, they were used for mRNA purification and library construction with the Truseq™ RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA) following the manufacturer's instructions.
Subsequently, they were used to screen 32 accessions, and detected an average of 8.25 alleles and from 3 to 15 alleles per locus (Table 2).
Subsequently they were used to anchor SSR-based maps in A. fistulosum[ 12] to physical chromosomes, and more recently to assign many more onion EST-derived anchor markers used in A. fistulosum maps [ 14].
After 1 week the micropellets were quickly frozen or embedded in paraffin or included in OCT freezing medium and subsequently they were used for RNA isolation or for histological and immunohistochemical stainings.
We investigated current practices of communicating abnormal Pap results to evaluate women's reactions and determine the sources of information they use subsequently.
