Subsequently, the expression patterns of preferentially expressed genes putatively involved in mescaline production were examined and validated by qRT-PCR.
Subsequently, the expression patterns of 10 candidate differentially expressed genes (DEGs) involved in the flavonoid metabolic pathway were examined, and flavonoids were qualitatively and quantitatively analysed.
Subsequently, the expression of eight of the annotated differentially expressed genes (DEGs) was characterized in the two varieties in response to a variety of abiotic stresses.
Subsequently, the expression of all components of the mini-replicon, in each condition, was evaluated by western blotting, except that of NS2, for which no good commercial antibody is available (Fig. 1b).
Subsequently, the expression of the DC maturation factors for this lipid composition was predicted and tested in vitro; the acquired maturation responses corresponded well with the predicted ones.
Subsequently, the expression of mIL-12 in the engineered B. longum was identified in vitro by western blot and enzyme-linked immunosorbent assay (ELISA) after l-arabinose induction.
Its expression was initially found throughout neuroectoderm at E7.5; subsequently the expression became high in rostral brain and caudal neuropore regions and low in hindbrain and spinal cord regions.
Subsequently, the expression of genes known to be involved in xylose fermentation (Additional file 1: Table S1) was analyzed.
Subsequently, the expression levels of the proliferation marker Ki-67 were evaluated by immunoblotting.
Subsequently, the expression levels of TIEG1, Runx2, osteocalcin, osteopontin, osterix and bone sialoprotein were determined via real-time PCR.
Subsequently, the expression of SlCXE10 was normalized to geometric means of this reference and the normalized gene expression was then calculated with Q-Gene software [42].
