Subsequently, the concentration of glucose, yeast extract, and MgSO4 were optimized using the orthogonal experiment design.
Subsequently, the concentration of sucrose, yeast extract and mineral sources were optimized using the orthogonal array method in two stages.
Subsequently, the concentration of the probe present in the homogenates was extrapolated from the calibration curves made with the standard probe, using the software Prism 5 (GraphPad Software Inc., La Jolla, California, USA).
Subsequently, the concentration of the elements present at trace levels (μg g-1) was determined by means of Instrumental Neutron Activation Analysis (INAA) for comparisons with obsidians of known provenance and well-studied micro-elemental composition, in order to identify the geographical origin of the obsidian used in this Middle Ages mosaic.
Subsequently, the concentration of the TaqMan probe was optimized.
Subsequently, the concentration of infectious virus in each sample was determined by plaque assays.
Subsequently, the concentration of VEGF in cell lysates was quantified using the log-log regression equation of the standard calibration curve (y = 0.0099x0.6137, R = 0.996).
Subsequently, the concentration of sHA in the flow-through is analysed by an ELISA-like assay that employs a HA-binding protein.
Subsequently, the concentration of the enriched library pool was measured by qPCR (Ion Library Quantitation Kit) and sequenced on the Ion PGM sequencer using a 316v2 or 318v2 chip and the Ion PGM Sequencing 400 Kit protocol.
Subsequently, the concentration of P can be determined from PT = 3ATP + 2 ADP + AMP + P. The relationship given in Eq. (5) dictates the decomposition of the stoichiometric matrix into two blocks, given by Eq. (8): 8 in which N is decomposed into blocks of N R, which is the reduced stoichiometry matrix, and N 0 [ 5, 6].
