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Subsequently, termination of the reaction was carried out at 72°C for five minutes followed by storage of the samples at 4°C.
Subsequently, termination of the reaction was carried out at 72°C for 10 minutes, followed by storage of the samples at 4°C.
Subsequently, termination of the reaction was carried out at 72°C for 10 min followed by storage of the samples at 4°C. 20 tumour-free (naive) mice were analysed as negative controls.
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Subsequently, the rats were equipped with a femoral catheter and arterial blood pressure was measured via transducers (Grass Instruments, Quincy, USA) connected to a polygraph (Hellige, Freiburg, Germany) four hours after termination of anesthesia.
Age and weight were 3.2 ± 2.9 months and 3.8 ± 0.5 kg for group 1, and 6.9 ± 1.4 months and 5.8 ± 1.9 kg for group 2. Two hundred μ l heparinized blood samples were obtained from an jugular bulb catheter before the induction of CPB, at 1 h (immediately after CPB termination), and subsequently at 2, 3 and 4 h postoperatively.
Our report describes a novel MR mutation in a patient with PHA, and this mutation was found to eliminate a splice acceptor site, which results in exon 7 skipping and subsequently in premature termination in exon 8.
Our observations suggest that oil quality improved during cell division; and subsequently plummeted following the termination of cell division (initiation of fatty acid accumulation).
It is assumed that the GC-rich parts of the synthesized ssDNA are forming stable secondary structures during the emulsion PCR process, which subsequently leads to a termination of the PCR reaction and only the non-folded part of the DNA fragment being amplified.
Today's deal means that KKR will no longer be taking a stake in Pandora, and Pandora will subsequently pay KKR a $22.5 million termination fee.
However, there also were rumors that SiriusXM would step in as a strategic investor to acquire a "substantial minority investment in the company". Today's deal means that KKR will no longer be taking a stake in Pandora, and Pandora will subsequently pay KKR a $22.5 million termination fee.
Based on their overlapping gene structure and transcriptional initiation and termination sites, we subsequently cloned promoters of miR-433 and miR-127.
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