Despite their noted advantages over mono-method designs, mixed method designs that start with quantitative data and subsequently supplement it with qualitative insights may not achieve the dominance that many have supposed and desired.
The Apollo tender offer is being made pursuant to a tender offer statement on Schedule TO filed by affiliates of Apollo Global Management, LLC with the SEC on March 13 , 2012as subsequently supplemented and amended.
Bacteria were sonicated in ice cold buffer A (20 mM Na2HPO4, 10 mM imidazole, 0.5 M NaCl, pH 7.4), and subsequently supplemented with lysozyme, protease inhibitors (Roche) and 20 mM beta-mercaptoethanol (final concentration).
GNS cells were transduced (MOI = 10) for 1 h and subsequently supplemented with fresh media.
Crude extracts are generated from cultured cells and depleted of endogenous DNA and mRNA, and the lysate is subsequently supplemented with energy components and free amino acids.
This basic solution was autoclaved and subsequently supplemented with 0.12 g of MgSO4.H2O, trace elements (mg/L): 6.0 FeSO4.7H2.7 2.0 CaCO3, 2.0 ZnSO4.H2O, 1.16 MnSO4.H2O, 0.37 CoSO4.7H2O, 0.33 CuSO4.5H2O, 0.08 H3BO3 (all filter-sterilized).
The medium jar was filled with 20 l autoclaved YNB without amino acids prior to autoclaving and was subsequently supplemented with 2% filter-sterilized glucose.
Finally, consider the scenario in which the analysis cohort has been directly genotyped only for variants on the GWAS chip, but are subsequently supplemented with imputation of untyped variants present in the reference panel.
Zn2+ imaging was carried out as described previously [ 19], with perifusion of the infected beta cell with buffer subsequently supplemented with 50 μmol/l TPEN and then 5 μmol/l pyrithione/100 μmol/l ZnCl2 (ZnPyr) (Fig. 1a, b).
