Sentence examples for subsequently supplemented from inspiring English sources

The Apollo tender offer is being made pursuant to a tender offer statement on Schedule TO filed by affiliates of Apollo Global Management, LLC with the SEC on March 13 , 2012as subsequently supplemented and amended.

Bacteria were sonicated in ice cold buffer A (20 mM Na2HPO4, 10 mM imidazole, 0.5 M NaCl, pH 7.4), and subsequently supplemented with lysozyme, protease inhibitors (Roche) and 20 mM beta-mercaptoethanol (final concentration).

GNS cells were transduced (MOI = 10) for 1 h and subsequently supplemented with fresh media.

Crude extracts are generated from cultured cells and depleted of endogenous DNA and mRNA, and the lysate is subsequently supplemented with energy components and free amino acids.

The medium jar was filled with 20 l autoclaved YNB without amino acids prior to autoclaving and was subsequently supplemented with 2% filter-sterilized glucose.

Zn2+ imaging was carried out as described previously [ 19], with perifusion of the infected beta cell with buffer subsequently supplemented with 50 μmol/l TPEN and then 5 μmol/l pyrithione/100 μmol/l ZnCl2 (ZnPyr) (Fig.  1a, b).

Despite their noted advantages over mono-method designs, mixed method designs that start with quantitative data and subsequently supplement it with qualitative insights may not achieve the dominance that many have supposed and desired.

Subsequently, supplementing soybean meal and corn meal with optimum concentrations determined by another RSM analysis produced an 8.8-fold increase.

We have deployed our QSAR prediction models via MHCPred [16]; subsequently supplementing this with sophisticated models of antigen presentation [17]; deployed via EpiJen [18].

The subject was diagnosed with adrenocortical insufficiency with elevated plasma ACTH at age 1 after a hypoglycemic seizure and was subsequently pharmacologically supplemented with glucocorticoids.

Next, the chips were primed in RPMI-1640 for 30 minutes and subsequently in supplemented RPMI-1640 for at least 30 minutes before seeding of cells.