Sentence examples for subsequently sequence from inspiring English sources

Subsequently, sequence analyses of the hypervariable repeat region was performed using the in-house sequence analysis software described previously [ 36].

We performed genomic PCR as described above to amplify and subsequently sequence 11,313 bp [GenBank: HM454273] that are 99.8% identical to the p2[4Co63] sequence.

We also performed PCR to amplify and subsequently sequence an ≈460-bp portion of the I. scapularis 16S rRNA gene using primers 16S +1 and 16S –16S20 ).

Using the same set of degenerate primers as for partial sequencing, initial sequences were obtained using the protocol described above and subsequently sequence gaps closed by primer walking.

Subsequently, sequence reads were assembled de novo using IDBA-tran v1.1.1 (Peng et al. 2013) and CLC Genomics Workbench v5.5.x (CLC bio, Aarhus, Denmark).

Subsequently, sequence data from additional genomes were collected from public databases including TBestDB [ 44], NCBI http://www.ncbi.nlm.nih.gov, TIGR http://www.jcvi.org, Kazusa http://bacteria.kazusa.or.jp/cyanobase and UniProt http://www.uniprot.org.org

Ten subclones were selected for each gene and subsequently sequenced using an ABI 3730 DNA sequencer (Applied Biosystems).

We confirmed the ViroChip's finding by subsequently sequencing this virus's genome.

These second round PCR products were subsequently sequenced.

Several clones were analysed by PCR using the same primers (Fig. 1b) and subsequently sequenced.

The PCR product was cloned into the pGEM-T (Promega, USA) and subsequently sequenced.