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Subsequently, sections of the 3D histology image were reoriented manually to align with the anatomical slice of the prostate on the T2w MRI (that was previously coregistered to the 18F-MK-9470 PET data set).
Subsequently, sections of the tumor blocks were stained with hematoxylin and eosin (H&E), Ki67, or PARP antibody (1 500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA) by the pathology core facility at Fox Chase Cancer Center.
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Subsequently, Section III covers the experimental procedure of DNA work, microscope instrumentation, data collection through imaging acquisition, data comprehension, and evaluation.
Subsequently, sections were incubated overnight in a mixture of rabbit polyclonal anti-Agrin with mouse monoclonal anti-Glial Fibrillary Acidic Protein (GFAP; Cymbus Biotechnology LTD, Chemicon International, CA, USA).
Subsequently, sections were incubated in blocking solution consisting of 5% BSA and 10% (v/v) normal goat serum in PBS at room temperature for 1 hour.
Subsequently, sections were incubated in reaction medium (consisting of 6.5 ml iso-ompa, 5 mg acetyl-thiocholine-iodine, 0.5 ml 1 M sodium citrate, 1.0 ml 30 mM copper II)sulphate, 1.0 ml distilled water and 1.0 ml 5 mM potassium ferricyanide) at 37°C for 6 h to visualise acetylcholinesterase activity.
Cut sections of the specimens were subsequently impregnated and photographed.
A replica of the most important sections of the cave was subsequently created nearby, and opened in 1983 as Lascaux II.
Subsequently, sections were counterstained with hematoxylin.
Subsequently, trial sections of 30 m long and 3 m wide (left side of Fig. 11) were constructed to assess the feasibility of using the double layered technique in practice.
Subsequently, cross sections of 5 μm thickness were fixed with 4% (wt/vol).
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