Sentence examples for subsequently sections from inspiring English sources

Subsequently, sections were counterstained with hematoxylin.

Subsequently sections were incubated for 60 minutes with polyclonal anti-CTGF primary antibody (1∶500; Santa Cruz, Santa Cruz, USA).

Subsequently, sections were incubated overnight at 4°C in the presence of the primary antibody diluted in PBS supplemented with 1.5% blocking serum.

Subsequently, sections were postfixed in 4% paraformaldehyde in 0.12 M phosphate buffer for 15 min at RT and rinsed in PBS.

Subsequently sections were rinsed (3×10 min) with TBS-Tween-20 and briefly with water and finally cover-slipped using Vectashield water-based mounting medium.

Subsequently, sections were washed with NaPBS and incubated at room temperature with biotinylated donkey anti-rabbit IgG (Amersham Biosciences, Sunnyvale, CA; 1∶500).

Subsequently, sections were digested with Proteinase K for 1 hr with gentle agitation at 37°C and digestion was stopped by washing in running water.

Subsequently, sections were deparaffinized and rehydrated.

Subsequently, sections were counterstained with hematoxylin and then dehydrated.

Subsequently, sections were counterstained with hematoxylin, dehydrated and mounted.

Subsequently, sections were counterstained with haematoxylin, dehydrated, and covered.