Sentence examples for subsequently second from inspiring English sources

Certainly, more people would know of her as the mistress and subsequently second wife of Kingsley Amis than could name any of her 12 novels, but if the Amis connection alone draws readers to her life story it will have been no disadvantage to her.

Subsequently, second strand cDNA was synthesized using RNase H (Invitrogen) and DNA polymerase (Invitrogen).

Subsequently, second strand cDNA was synthesized using RNase H and DNA polymerase.

8 Subsequently, second generation non-hysteroscopic techniques have been developed, which are easier to perform.

Subsequently, second set of miRNA-specific primers was used to amplify each miRNA according to the manufacturer's recommendations.

Although it was proposed that TGF- β signaling is enhanced via clathrin-coated pit internalization and the caveolae/Caveolin-1 dependent route would serve for dampening of signaling, the presented work demonstrates that first, Caveolin-1 is required for activation of AKT by TGF- β and subsequently second, that reduced Caveolin-1 levels shift the physiological hepatocyte response towards apoptosis.

Her scheduled second (and subsequently third) immunization was cancelled due to Bell's palsy affecting Case 1.

Subsequently, first-strand cDNA was synthesized from total RNA (2 µg) using oligo dT) primers following the manufacturer's instructions of PrimeScript™ RT-PCR Kit (TaKaRa, Japan).

Subsequently, second-strand cDNA was produced using DNA polymerase I and RNase H.

Subsequently, second-level coding was conducted by collapsing initial codes into broader categories, identifying category properties and establishing the relationships between categories [ 30].

At this time, the number of chromosomes was restored from six to 12. Subsequently, first and second cleavages occurred.