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This transcript can be subsequently reverse transcribed by Ty and integrated into the genome resulting in His+ prototrophic cells in a strain containing chromosomal his3 deletion [49].
(4) Middle Miocene basin morphology in central Hokkaido was successfully reproduced by assuming hybrid fault motions; these included initially right-lateral and subsequently reverse motions in the longitudinal fault zone.
Total RNA was isolated from cells by TRIZOL Reagent (Invitrogen) and subsequently reverse transcribed using an oligo-dT primer (Invitrogen) and a SuperscriptII first strand synthesis kit (Invitrogen).
If subjects were to select button A on every choice in the RO task, they would experience a temporary decrease in earned reward that would subsequently reverse to produce the maximum average return (Figure 1B).
Standardised amounts (200 ng) of template were subsequently reverse transcribed using the TaqMan Reverse Transcription Reagents kit (Applied Biosystems) in 20 µl reactions following the recommendations of the manufacturer.
mRNA was subsequently reverse transcribed using SuperScript II Reverse Transcriptase (Invitrogen, 18064) and oligo d(T) primers, and subsequently used in 5'RACE, RT-PCR or real time PCR.
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Justices Blackmun and Stevens subsequently reversed their views and Justice Brennan modified his.
The administration subsequently reversed its policies or discontinued them in the face of criticism.
Wilson subsequently reversed his opinion of Lenin, and then compared Lincoln and Lenin, as types of the modern dictator, in the introduction to "Patriotic Gore".
Ms. Pelosi's test flows directly from a fierce conflict over national security policies that Mr. Cheney backed as vice president and were subsequently reversed by President Obama.
Low point: Making the wrong decision in not proceeding against a police officer over an alleged racist incident, a decision she subsequently reversed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com