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Their use was subsequently restricted in several other countries.
De Bruyne's starts were subsequently restricted to three League Cup games, and in the last week of December he told the club that he wanted to leave.
Population allocation in LUISA is subsequently restricted by three factors.
For the P comI -GFP fusion the promotor region of comI was amplified using the primer pair comI13f_KpnI/comI13r_ClaI. The PCR product was subsequently restricted with ClaI and KpnI and ligated into the likewise restricted vector pMUTIN-GFP+, resulting in plasmid pMUTIN-comI.
Analysis was subsequently restricted to the remaining 23,496 CpG loci (12,956 genes), 18,742 located within CpG islands and 4,754 located outside of these regions.
Using a genome-wide scan and 225 anchored microsatellite markers, we placed the four bulldog mutations on zebrafish chromosome 7 and subsequently restricted the critical interval to 0.33 cM between markers Z13880 and Z13936 (Figure 1A).
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Additionally, ankle braces may cause a tighter fit in the boot shaft region and subsequently restrict motion at the ankle joint.
Chronic stress may trigger migraine attacks [8, 9] or induce CM [11, 33], subsequently restricting or preventing participation in social or work related activities [35].
That his handlers would subsequently restrict access seemed more than coincidental.
These findings reveal a new and fundamental role for Sdf1a in the process of fin regeneration, first as a possible mediator of the Fgf proliferative activity in the blastema formation, and then in the amplification of a negative feedback loop initiated by Wnt5b which subsequently restricts Fgf20a activity to allow proper regeneration.
Administrative data are also limited in the information recorded, subsequently restricting the research questions that can be answered.
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