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We find a threshold which strangler germination rates must exceed for selection to favor the evolution of strangling, regardless of how effectively hemiepiphytic stranglers may subsequently replace their hosts.
For site-directed mutagenesis, the phage λ-based homologous recombination system (Red/ET counterselection Bac modification kit; GeneBridges, Heidelberg, Germany) was used to introduce an rpsL-neo cassette into acrR or marR of strain JA300 and to subsequently replace the cassette with an appropriate DNA fragment.
The two approaches are compared by incorporating their resulting sample designs in Monte Carlo simulation (MCS) for uncertainty propagation analysis, and then, by employing the sample designs in the selection of the training set for constructing non-intrusive polynomial chaos expansion (NIPCE) meta-models which subsequently replace the original full model in MCSs.
We tested this concept in our study by asking whether BM-transplanted progenitor cells from a healthy donor might reconstitute the recipients BM and subsequently replace dysfunctional endothelium in an animal model with haemodynamic evidence of chronic endothelial dysfunction.
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These policy changes involved replacement of chloroquine (CQ) with sulphadoxine-pyrimethamine (SP), which was subsequently replaced by artemether-lumefantrine (ALu) [ 49, 50].
Henriksson was subsequently replaced in March this year.
Destroyed in 1891, the canvas was subsequently replaced.
Mr. Yang was subsequently replaced as chief executive.
Islam subsequently replaced Christianity in parts of the region.
Moore was subsequently replaced, he alleged, by a less experienced member of staff.
The dictatorship subsequently replaced the European dream with the American dream".
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