Sentence examples for subsequently reconstituted from inspiring English sources
In our attempts to reconstruct these events in vitro we have previously linked in tandem two copies of the C-terminal half barrel HisF-C of imidazole glycerol phosphate synthase from Thermotoga maritima and subsequently reconstituted in the fusion construct HisF-CC a salt bridge cluster present in wild-type HisF.
He subsequently reconstituted his organisation as the third incarnation of the Alouettes.
EYFP and ECFP images were subsequently reconstituted by linear unmixing of the wavelength series.
Hspol γ is a heterotrimeric enzyme comprising one catalytic and two accessory subunits (αβ2) [33], [33]; the α and dimeric β subunits can be expressed in heterologous systems and purified independently [15], [18], and the holoenzyme is subsequently reconstituted in vitro.
To remove potential growth inhibitors or enhancers, serum samples were dialysed against PBS using 50 kDa MWCO microdialysis tubes (2051, Chemicon, Temecula, CA, USA) and subsequently reconstituted to original volume using centrifugal concentration tubes (100 kDa MWCO; Pall Corp ,Ann Arbor, MI, USA) prior to testing in GIAs [38].
The latter were subsequently reconstituted in liposomes.
Both subdomains were genetically fused to the HaloTag protein, a self-labeling enzyme, enabling bioconjugation with chloroalkane-modified DNAs and subsequently reconstituting BM3 activity by DNA-mediated assembly.
Because of the difficulties with air sensitivity and cluster lability, radical SAM enzymes are often isolated as apo-enzymes, under aerobic or anaerobic conditions, and subsequently chemically reconstituted in vitro with iron and sulfide under anaerobic conditions to generate the active enzymes.
Subsequently, these keratinocytes reconstituted an epithelial tissue architecture resembling native epidermis and expressing characteristic epidermal differentiation markers (18- 19, 1, 9).
Subsequently, the reconstituted formerly glycosylated peptides from each sample were analyzed by three LC-MS/MS technical replicates.
95 The administration and targeted cellular delivery of specific miRNAs via EMV-like vehicles prepared from natural sources and subsequently chemically modified or reconstituted in vitro is under intense investigation and discussion.
