Sentence examples for subsequently primary from inspiring English sources

This paper outlines results from an experimental imaging investigation into the effects of fuel properties, temperature and pressure conditions on the extent of cavitation, flash boiling and, subsequently, primary break-up.

Subsequently, primary mouse monoclonal antibodies against JAM-A (clone M.Ab.F11, AbD Serotec, Düsseldorf, Germany), zonula occludens-1 (ZO-1, clone ZO-1-1A12 ZO-1-1A12 ZO-1-1A12co, CA) or occludin (clone OC-3F10, Zymed) were incubated at 4°C overnight.

Probes were blocked and permeabilized with 7% normal goat serum and 0.3% triton-X100 in 0.01 mol/l PBS for 2 h at RT. Subsequently primary antibodies (dilution: 1∶200 in 2% bovine serum albumin with 0.05% acid and 0.1% triton X-100 in 0.01 mol/l PBS) against cleaved caspase-3 were incubated overnight at RT.

Subsequently, primary schools within these municipalities were requested to participate.

Subsequently, primary suspensions were diluted to obtain the required cells/ml for the infection experiment.

Subsequently, primary and secondary antibodies were applied in GDB buffer, as described previously.

Subsequently, the primary plastids were laterally transferred to several eukaryotic lineages as result of a eukaryote engulfing another eukaryote carrying a primary plastid, then giving rise to secondary plastids, or even engulfed eukaryotes harboring secondary plastids (a tertiary plastid).

Subsequently, the primary outcome measure used to assess treatment effectiveness has been survival.

Subsequently, the primary and a secondary observer performed a blinded reanalysis of 50% of all the echocardiographic measurements.

Subsequently, the primary heart tube is elongated by recruiting cells that are generated from the second heart field.

Subsequently, the primary organizational climate of terminal operation industry, namely, management system, awards and motivation, transformation leadership, and laissez-faire leadership, were yielded, and the employees' job satisfaction were used as explanation variables in this study.