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SMRTbell template libraries were subsequently prepared using the commercial Template Preparation Kit from Pacific Biosciences Inc. and involved the sequential steps of DNA end repair, adapter ligation and exonuclease digestion of incompletely ligated products.
The samples were subsequently prepared according to a standardized preparation protocol, mentioned below.
Transparent ZnO/epoxy nanocomposites were subsequently prepared from transparent epoxy (EP-400) and as-prepared ZnO nanoparticles via in situ polymerization.
Realising that they had only faced a series of probes the previous night, rather than a major attack, and as these actions may have been in preparation for a heavier attack, a vigorous defence was subsequently prepared by the Australians with the reserve companies moved into blocking positions to strengthen the forward defences.
Alan Todd and Gill Tuck subsequently prepared and curated the data.
The corresponding catalyst was subsequently prepared by a copper ion-exchange method.
ONC nanofibrils were firstly extracted from bamboo fibers and subsequently prepared by applying TEMPO oxidation.
PIL/IL ion-gel membranes were subsequently prepared and tested for CO2/light gas permeation.
The chalcones with the best docking scores obeying the Lipinski rule of five were subsequently prepared by base-catalyzed aldol reactions.
The polymers were subsequently prepared from the bis-oxindole and bis-isatin in good yields by simply refluxing a toluene solution of the monomers in the presence of p-toluene sulphonic acid monohydrate.
Well-defined biocleavable comb-shaped vectors consisting of nonionic dextran backbones and disulfide-linked cationic P DMAEMA) side chains were subsequently prepared via ATRP for highly efficient gene delivery.
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