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Subsequently, portions of the 5'-UTR of phzA1-G1 were taken to predict three suboptimal structures, and the free energies of optimal and suboptimal structures were calculated with mfold [50].
Subsequently, portions of 40 ml of the preculture of B. subtilis were exposed to seven different temperature regimes in 250-ml bottles in a reciprocal-shaking water bath at 65°C.
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For the chips with higher anti-EpCAM coverages (i.e., 5 and 10%), their cell-release performances were less impressive due to the fact that the cells released from their initially captured locations were subsequently "recaptured" on a later portion of the microchannel.
The indictment said the high-ranking Fifa official identified on Monday as Valcke caused $10m to be wired to accounts controlled by Warner, and Warner subsequently diverted portions of the money for his personal use and to personal accounts.
Subsequently, a portion was freeze dried, dissected free from visible connective tissue and blood, powdered and extracted in 0.5 M perchloric acid containing 1 mM EDTA.
Subsequently, a portion (300 g) of the dried marc was boiled in distilled water (2 L) for 3 h, then filtered and dried to give 95.1 g of the hot aqueous extract (AqE, 3.17% yield).
Subsequently, a portion of this intermediate (6.5 mg, 7.9 μmol) was dissolved in DMF (1 ml) and a solution of Dig-Amcap-OSu (5.2 mg, 7.9 μmol) in DMF (1 ml) and 1% triethylamine in DMF (2 ml) were added and the mixture was tumbled for 16 hours.
Students who are particularly metacognitive or self-aware recognized deficits in comprehension upon returning to the primary task and subsequently re-read portions of the article after interruption.
Subsequently, 100 ml portions of 0.3 M (NH4 2HPO4 solutions were poured drop wise into the Ca(NO3 2 solutions to get the final Ca/P ratio equal to 1.67.
Subsequently, the three portions were fixed using the conventional FFPE protocol, the recently introduced HOPE-fixation, and cryo-conservation method.
Since then, the government has helped recapitalize A.I.G. and subsequently sold a portion of its shares in the insurer.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com