Cells were subsequently labeled with FITC-labeled goat anti-mouse IgG (Sigma-Aldrich, USA) at 1 : 400 dilution for 1 h at 37°C.
This expectation was precisely matched in a double-labelling experiment, in which AChRs were subsequently labeled with two distinctly coloured BGT species at different time points in the presence or absence of silMyoVa (Fig. 6).
The amplified mRNA was subsequently labeled using the IVT Labeling Kit (Affymetrix), to produce biotin-labeled mRNA suitable for hybridizing to GeneChip Probe Arrays (Affymetrix U133 Plus 2.0).
Thus, glycans synthesized and trafficked to the cell surface between 73 and 76 hpf and subsequently labeled with DIFO-555 could be distinguished from the DIFO-488-labeled glycans that were synthesized during the first three days of development.
However, these Chinese writers, who were subsequently labeled nanlai zuojia ("writers who came to the south"), had little concern about the development of Hong Kong literature.
enNTS1 was subsequently labeled with 13CH3-methionine in E. coli and purified to homogeneity in the absence of bound ligands.
Each individual aliquot is subsequently labeled with a 2-D barcode encrypted for each patient and sample type to provide full traceability of the samples.
Following blocking of free protein thiols with N-ethylmaleimide, protein sulfenic acids were reduced by arsenite to free thiol groups that were subsequently labeled with biotin-maleimide.
Three lead compounds were subsequently labeled with carbon-11 or fluorine-18 to directly assess their potency in a direct radioactive competitive binding assay ng both α-synuclein fibrils and tissue homogenates from Alzheimer's disease (AD) cases.
Cetuximab was modified using the chelator desferal (Df; desferrioxamine B, Novartis Pharma BV, Arnhem, The Netherlands) and subsequently labeled with 89Zr as previously described by Cohen et al. [8].
An Asn23Cys mutant of the B domain from SPA was expressed in Escherichia coli and subsequently labeled at the introduced unique thiol and at an amino group, using N-iodoacetyl-N′- 5-sulfo-1-naphthyl)ethyleN-iodoacetyl-N′- 5-sulfo-1-naphthylmidyl 6-(N-iodoacetyl-N′- 5-sulfo-1-naphthyll)amino)hexanoate N-iodoacetyl-N′- 5-sulfo-1-naphthyl
