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After incubation, plates were placed on ice and to quench the fluorescence of non-phagocytosed yeast, an equal volume of ice-cold trypan blue (250 µg/ml in 0.1 M citrate buffer, pH 4.0; Fluka) was added to each well, incubated for 1m on ice and the cover slips were subsequently inverted and mounted onto slides for viewing with an inverted microscope (200×).
For percent area fraction analysis, images were converted to binary images and subsequently inverted.
In ionospheric tomography the TEC data are analyzed and, subsequently, inverted using a mathematical reconstruction algorithm that yields three-dimensional images of electron density throughout the regions of the satellite-to-ground ray-path intersections.
The NARMA-L2 Neural Network is trained off-line to identify the forward dynamics of the UAV model with the redefined output, which is subsequently inverted to force the real output to approximately track a command input.
Analytical solutions of concentrations in the aquitards and aquifer and mass transported between the aquifer and upper or lower aquitard are obtained in the Laplace domain, and are subsequently inverted numerically to yield results in the real time domain (the Zhan method).
Coverslips were washed three times in 3× PBS for 10 min and subsequently inverted on 250 µl anti-DANGER1a conjugated to Alexa 488© fluorophores from Molecular Probes in a wet chamber overnight at 4°C.
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Subsequently, individual z scores were summed, after inverting HDL-cholesterol z scores and averaging systolic and diastolic blood pressure z scores, and divided by the number of variables included.
These structures have subsequently been inverted to form arc-normal oriented zones of tear faulting that accommodate laterally variable displacement along inversion faults and connected thrust structures.
Cell images were collected following detachment as grayscale images and subsequently color inverted for better visualization contrast.
The random effect is subsequently modeled with an inverted beta distribution, resulting in an inverted beta binomial model for the observed count data.
Cells on the bottom side were subsequently counted under an inverted microscope.
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