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DNA from all donors was stored at the time of assay, and we were therefore able to genotype all donors subsequently for both polymorphisms (V249I and T280M) to establish whether CX3CR1 genotype affected response to CX3CL1.
The muscle samples were immediately frozen in liquid nitrogen and stored for protein extraction for samples from pre- and 12 weeks post-exercise program (chronic exercise study) and subsequently for both RNA and protein extraction (acute exercise study: for samples pre- and 1-h and 7 days post-exercise).
Earlier investigations for UVR-filtering IOLs by Charman, and those published subsequently for both types of IOLs by the authors opposing the blue light-filtering IOL, used an action spectra for photoentrainment with a peak of 460 nm [ 3, 7, 14, 73].
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The approach subsequently allows for both the increased dependability of the composite service and increase trust from the end-user.
Sixteen ml of amniotic fluid (AF) was cultured using the in situ method in BD Falcon Culture slides (VWR, Amsterdam, The Netherlands) and subsequently used for both GTG-banded karyotyping and fluorescence in situ hybridization (FISH).
These sequences were subsequently used for both phylogenetic and population genetic analyses.
Therefore, all analyses were subsequently adjusted for both ethnicity and smoking by including in logistic regression models as covariates.
A keratoplasty procedure was subsequently performed for both groups (15 cross-linked donor keratoplasties and 15 standard untreated donor keratoplasties).
If an opportunity for HH was given for two reasons (=indications) simultaneously, it was documented as one opportunity, but subsequently analyzed for both indications.
An entire cDNA fragment for each of the putative P450 genes was subsequently amplified for both ALHF and aabys house flies by PCR using the primer pair (Table 1) synthesized based on the respective 5' and 3' end sequences of each gene.
An in silico global expression analysis was subsequently performed for both gene sets in response to specific stimuli and in selected mutants to identify conditions that induce differential expression of the genes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com