Sentence examples for subsequently first from inspiring English sources

George Washington, also called Father of His Country (born February 22 [February 11, Old Style], 1732, Westmoreland county, Virginia [U.S.] died December 14 , 1799 Mount Vernon, Virginia, U.S ., American general and commander in chief of the colonial armies in the American Revolution (1775 83) and subsequently first president of the United States (1789 97).

From 1963 to 1968, he held the positions of second, and subsequently first, secretary of the Gorky Komsomol, and later became Chairman of the Committee of Youth Organisations, which he held for 12 years.

At this time, the number of chromosomes was restored from six to 12. Subsequently, first and second cleavages occurred.

Subsequently, first-strand cDNA was synthesized from total RNA (2 µg) using oligo dT) primers following the manufacturer's instructions of PrimeScript™ RT-PCR Kit (TaKaRa, Japan).

Her scheduled second (and subsequently third) immunization was cancelled due to Bell's palsy affecting Case 1.

Subsequently, first-strand cDNA synthesis and qPCR were performed using the PrimeScript™ RT-PCR Kit (TaKaRa) and the SYBR Premix Ex Taq II™ kit (TaKaRa).

The simulated datasets were re-estimated subsequently (first-order conditional estimation method with interaction) using (1) a model including the covariate effect (full model) and (2) a model not including the covariate effect (reduced model).

Subsequently, first-pass quality control and genotype calling from the raw data in the form of CEL files were implemented with Affymetrix Power Tools v1.16.0 (APT) software using the Axiom GT1 algorithm.

Subsequently, second strand cDNA was synthesized using RNase H (Invitrogen) and DNA polymerase (Invitrogen).

Subsequently, second strand cDNA was synthesized using RNase H and DNA polymerase.

8 Subsequently, second generation non-hysteroscopic techniques have been developed, which are easier to perform.