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These PBMC were subsequently, enumerated, cryo-preserved and stored in liquid nitrogen in Zimbabwe prior to freighting to Edinburgh in dry shippers for assay.
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The main principle of the bottom-up methods is to consider the reflection and attenuation at the impedance mismatch points and subsequently enumerate, simplify, and classify all paths between the signal transmitter and the information receiver based on the actual network topology.
This was done by first collapsing the obtained assignments (by a given method) at or below the desired taxonomic level of comparison and subsequently enumerating the same.
They construct a bipartite metabolic network consisting of compound and reaction nodes and subsequently enumerate all possible paths between a source (d-glucose) and a target compound (pyruvate) under certain constraints.
Moreover, the algorithm is readily parallelized using a divide-and-conquer strategy to split the problem into exclusive sub-problems using flux constraints and subsequently enumerate EFMs using as many computing nodes as available with minimal coordination effort.
Three distinct patterns of LC3B immunohistochemical staining were identified and enumerated in each tissue core (subsequently referred to as sections); (i) cytoplasmic, (ii) crescent or ring-like structures and (iii) large globule like structures.
Subsequently, all neighbors y ∈ M (x ) are enumerated to update Z { F (x ), F (y ) } if F (x ) ≠ F (y ).
Bacteriophage were propagated using the whole plate lysis method, enumerated on C. jejuni strains HPC5 (CP220) or NCTC12668 (CPt10) and subsequently stored at 4°C in SM buffer as described previously [ 10].
Therefore, we used micro-costing, which directly enumerates and subsequently values each input consumed for each particular participant.
This area was delimited with an electronic gate, which subsequently was used to enumerate leukaemia cells at the end of culture.
To this end, we used a recently developed ex vivo flow cytometry-based assay platform, which enables us to enumerate and subsequently deduce the drug/antibody-mediated lysis of primary CD138+ MM cells directly in bone marrow samples from the MM patients.
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