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Subsequently, diluted anti-BrdU peroxidase (dilution ratio of 1 100) was added to the cells and incubated at 20°C for 120 min.
HCV JFH-1 virus was subsequently diluted in 500 μL of DMEM complete medium (dilution fold = 1 2) (2 × 106 HCV RNA copies) and added to the cells.
The reaction mixture was subsequently diluted with water and extracted with EtOAc (2 × ).
Unlike income taxes, which are often front-loaded on the rich, then subsequently diluted, a value-added tax is paid by almost everybody.
200 ng of total RNA was reverse transcribed using the High Capacity cDNA Synthesis kit (Life Technologies) and cDNA was subsequently diluted 1 10 in nuclease-free water.
The results strongly suggest that NO formation occurs in near stoichiometric regions of the flame and is subsequently diluted with excess air.
The challenge microorganisms were grown to early stationary phase and subsequently diluted to reach initial inoculum levels of either 5 or 7 log cfu/slice.
Digested samples were subsequently diluted in 10 ml deionized water.
Samples were allowed to cool down and subsequently diluted 20 times using 0.014 M H2SO4.
Therefore, the samples of higher concentration of phenol were subsequently diluted with DDW.
All tested compounds were dissolved in DMSO and subsequently diluted in the culture medium before treatment of the cultured cells.
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CEO of Professional Science Editing for Scientists @ prosciediting.com