Sentence examples for subsequently concentrated from inspiring English sources
Individual DNA samples were pooled (50 ng per individual) and subsequently concentrated using a DNA concentrator Eppendorf 5301.
The fractions containing proteins (MBP-ΔN597 or MBP-ΔNCP69) were confirmed by SDS PAGE, and were subsequently concentrated using 10 kDa MWCO Vivaspin Sample Concentrators (Sartorius Stedim Biotech).
PolyA RNA was subsequently concentrated by ammonium acetate ethanol precipitation and concentrations were determined using the Qubit fluorometer (Invitrogen, Carlsbad, CA) and the Quant-iT RNA Assay Kit (Invitrogen, cat#Q33140).
Purified DBLs were subsequently concentrated by means of Macro- and Micro-sep concentrators.
The eluted and neutralized samples were combined, diluted to 20ml with MilliQ water and subsequently concentrated to the original starting volume using centrifugal concentration tubes [Vivaspin 20, Sartorius AG, Goettingen, Germany: Code no VS2021].
The protein was cleared of imidazole using dialysis with phosphate buffered saline and subsequently concentrated using a 9 kDa cut-off protein concentrator according to the manufacturer's instructions (Thermo Fisher Scientific, Rockford, IL, USA).
Refolding was attempted using the detergent dilution method that has been successful for refolding other helical membrane proteins [20,21]; the SDS concentration was diluted to below its cmc with 20 mM Tris buffer, pH 7.5 containing 0.1% DDM and subsequently concentrating the sample using a concentrator with a 100 kDa cut-off membrane (Millipore) to ∼ 100 μl.
Subsequently concentrating on the three-cushion game, he won 12 annual world championship tournaments (1936, 1940 44, 1947 52).
The paper gives an overview of the CMS tracker system and subsequently concentrates on the results from the sensor tests.
In the military sphere, Piłsudski, who had shown himself an accomplished military strategist in engineering the "Miracle at the Vistula", has been criticized by some for subsequently concentrating on personnel management and allegedly neglecting modernization of military strategy and equipment.
The task force would subsequently concentrate astride Route 16 on one of the major north-south supply routes 40 km north-east of Saigon, just east of Lai Khe in Binh Duong Province, in a new area of operations known as AO Surfers.
