Sentence examples for subsequently be fixed from inspiring English sources

Live stained cells could subsequently be fixed and stained with further antibodies.

Accumulation of deleterious mutations may subsequently be fixed by alleles acquired from other populations in which purifying selection had been maintained.

The media was removed from each well and cells subsequently were fixed in 4% formaldehyde in HBSS for 10 minutes at 37°C.

(When presented with a binary choice of either 9 or 15°, the analytical software selected 15° for each peptide in DLPC but 9° for each peptide in DOPC; the values subsequently were fixed).

Feeding experiments were performed after 6 months, and subsequently stomachs were fixed in paraformaldehyde and sections stained with hematoxylin and eosin; inflammatory cell infiltrates were scored as previously described (21).

Subsequently, cells were fixed and treated for immunofluorescence analysis.

Subsequently, slices were fixed overnight in 2% paraformaldehyde at 4°C.

Subsequently, cells were fixed and permeabilized using a commercial kit (BD Pharmingen) according to the manufacturer's instructions.

Subsequently, cells were fixed with 4% para-formaldehyde (Sigma) containing 5% Sucrose for 20 minutes at room temperature and washed twice with DPBS.

Subsequently, cells were fixed with 2% formalin for 10 min, and permeabilized with 0.1% Triton X-100 in PBS (1 min, room temperature).

Subsequently, DCs were fixed with 2% paraformaldehyde in PBS for 30 min at 4°C, and then stained with 20 µg/ml FITC-conjugated anti-mouse LAMP-2 (Pharmingen) for 2 hr at 4°C to stain lysosomes.