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Analogous to the lithography process on a conventional two-dimensional surface where substrates are aligned and exposed subsequently, batch fabrication approach can be utilized in the sidewall patterning process using the proposed 3-D mask.
After membrane purification and detergent extraction, Syt1 was subsequently "batch" purified with Ni-NTA resin (Qiagen) at 4 °C, followed by 1D4 antibody affinity purification, in 20 mM Tris (pH 8.0), 0.5 M NaCl, 10 mM CaCl2, 5% glycerol, 1% FOS-14 (or DDM), and protease inhibitors.
Subsequently, batch bioreactor cultivations were performed to demonstrate that cultures can be productively infected at a CCI of 4 × 10 cells/mL without any medium exchange, doubling the CCI that has been used previously with CR.pIX cells (2 × 10 cells/mL) [ 23].
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Moreover, the hydrolysates obtained after batch and fed-batch enzymatic hydrolysis has subsequently been fermented to ethanol, and an overall comparison between batch and fed-batch process has been presented.
Subsequently each batch was split into two sub-batches.
Subsequently, aerobic batch cultivation was performed in a 2.5-L bioreactor (Biostat; A. B. Braun Biotech International, Melsungen, Germany) at 30°C.
Subsequently, the batch process model parameters computed via the proposed R2R model parameter estimation scheme are used in a model predictive controller (MPC) within each batch to compute a set of optimal jacket temperatures for the production of crystals with a desired shape distribution in a batch crystallization process.
Subsequently, aerobic batch cultivations were performed in a 2.5-L bioreactor (Biostat; A. B. Braun Biotech International, Melsungen, Germany) at 30°C.
Cytokine concentrations of tumor necrosis factor-alpha (TNF-α), interleukin (IL -6, IL -6receptor antagonist (IL1RA), and IL-1-receptorasured IL1RAmples tandn at baseline and at 30, 60, 120, 240, and 480 minutes after LPS administration and subsequently analyzed batch-were with a Lumeasuredsay (Luminex Corporation, AusamplesX, USA) [ 18].
The reactor was run initially in continuous flow manner (CFM) for 120 days, with DO level 0.7 1.5 mg/L, without pH control, and run subsequently in sequencing batch manner (SBM) with DO level 7.0 8.0 mg/L.
Aliquots of 2 ml were stored at −20°C and subsequently assayed in batches.
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