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Reads that did not align were subsequently assembled de novo using the sequence assembly program MIRA [ 46] version 3.2.0 with the parameters "--job = denovo,genome,454,normal -SK mnr = yes 454_SETTINGS -LR wqf = no -notraceinfo -AS:epoq = no".
The pattern of molecules was subsequently assembled to align with the Illumina-based assembly to achieve sequence extension.
The donor transcription units were amplified with primers that introduced terminal BsaI restriction sites, which were subsequently assembled into the universal vector pYW0120 by Golden Gate assembly.
Paired-end reads were merged using FLASH36 and subsequently assembled in NEWBLER34.
Overlapping cosmids spanning a single pathway were initially sequenced separately and subsequently assembled into larger contigs.
This 2-helix polypeptide subsequently assembled into a typical 6-helix bundle.
The resistive central solenoid magnets, compensating coils and the newly designed in-vessel radial control coils will be subsequently assembled.
The computer subsequently assembled or compiled the programs and then executed them, and the results were then transmitted to a printer.
According to evidence in the case, the company subsequently assembled a panel of doctors to review reports it had received and determine which cases were likely to be osteonecrosis of the jaw.
Specifically, during the experimental activities, a prototype panel was first tested inside a climatic test room and subsequently assembled on mini cold rooms and evaluated under real summer environmental conditions in Ancona (Italy).
The method can also be applied to plates consisting of rectangular segments, like an L-shape plate, which sub-domains can be formulated using the computed shape functions and subsequently assembled in the usual finite element manner.
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