Plasma was subsequently aliquoted and immediately frozen at −80 °C for later analysis of [NO2−] as described previously (Wylie et al. 2013a, b).
Unfed plateau-phase cultures of Chinese hamster V79-cells were treated for 1 h with various amounts of adriamycin in the range between 0 and 10 micrograms ml-1 and subsequently either immediately trypsinized and plated to assay for survival, or reincubated in medium collected from replicate plateau-phase cultures and returned to the incubator for various periods of time before plating.
Moreover the CT scans obtained before and immediately after ablation and subsequently were reviewed by one experienced radiologist for the presence of gallbladder wall thickening of more than 2 mm, abnormal gallbladder wall enhancement, and pericholecystic fluid.
Subsequently the crystals were harvested and immediately flash-cooled in liquid nitrogen.
Each fraction was subsequently aliquoted into polypropylene cryovials and immediately frozen.
Subsequently, suction blister roofs were taken and immediately stored in liquid nitrogen.
Such new methods can be plugged dynamically into CellMissy, and will subsequently be immediately available to the user from the CellMissy interface.
And, the medical records for every one of the veterans in that area who were evacuated and subsequently relocated were immediately available to the VHA facilities and providers that took over their treatment and care management.
At the screening visit, 52 volunteers were examined and subsequently 15 were immediately excluded: in 8 cases for abnormalities in the clinical safety tests, in 3 cases for positive anti-PfCS102 antibodies, in 1 case for consent withdrawal, in 2 cases for recent travel in a malaria endemic country, and in 1 case for abnormal cardiovascular physical exam.
Subsequently, the supernatants were transferred to fresh tubes and immediately stored at –80°C.
Uber said in the post that it discovered a breach occurred in September 2014, and that it subsequently immediately changed its access protocols and began investigating.
