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Sections were taken every 7 µm, but only sections from 150 µm to 550 µm were subsequently affixed to glass slides for staining as these contained the articular cartilage.
PCL nanofiber coated TCPS was then placed in a vacuum overnight to insure removal of residual solvent [ 44], and then plasma treated (Harrick Plasma) to promote cellular attachment [ 36], and subsequently affixed to 24-well cell culture plates for cellular experiments.
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Subsequently, cells were washed twice in PBS and affixed to Superfrost (Fisher) microscope slides with 1 2 drops of SlowFade Gold (Invitrogen) to minimize loss of fluorescent signal.
Images were subsequently opened in the Metamorph software and a grid was affixed on the image defining three regions of interest (ROIs).
Electrodes were affixed.
was affixed here.
affixed to their names.
Affixed to fence b.
An assistant affixed another microphone.
fuel — affixed to the back.
JJ glowed, her smile affixed in place.
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