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Total RNA was extracted by using TRIzol method (Invitrogen, NY, USA) and complementary DNA was subsequently acquired from 1 μg of total RNA by reverse transcription as per the manufacturer's instructions (Bio-Rad, CA, USA).
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Other nonphotosynthetic eukaryotes may have subsequently acquired endosymbionts from photosynthetic eukaryotes to create secondary plastids (Howe et al. 2008; Keeling 2010).
This is compatible with the scenario that Haladaptatus originally had the A form and has subsequently acquired the B' form through HGT from another haloarchaeon.
He and his wife subsequently acquired a portfolio of 14 patents from a company called Firepond.
We applied an AEVSM to the monthly spring discharge records for Niangziguan Springs in China, from January 1959 to December 2009, and subsequently acquired the spring discharge distribution of extreme values.
He was commissioned in 2011 to create a series of images of contemporary laboratories to accompany an exhibition of work by Berenice Abbott, which were shown in the MIT Museum from May to December 2012 and subsequently acquired by the museum.
From this simple DNA ancestor, each NCLDV lineage could have subsequently acquired a large number of lineage-specific genes from a cellular source.
Either if already detectable from the beginning or subsequently acquired during the course of the disease, MCAM/MUC18 is significantly associated with poor prognosis and death (p < 0.001).
Thus, these two ORFs likely originated from a single leukocidin gene that subsequently acquired a nonsense mutation.
They reported that all the tumours of a single patient were derived from a common progenitor cell and subsequently acquired various additional genetic alterations.
Long Island Savings Bank converted from mutual ownership, went public, and was subsequently acquired by Astoria Federal Savings.
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