Nitrite was depleted after 12 days, and subsequently a second spike of 0.5 mM nitrite and 1 mM formate was added to the continuous culture.
Subsequently, a second procedure is described, which is a more rigorous approach able to identify liposome dependency for a drug that is less effective in a carrier system than it is in the free form.
Initially, a first order response surface model has been considered and steepest ascent method has been used to reach in the vicinity of optimal region of the response surface and subsequently, a second order response surface model with central composite design (CCD) has been used to take into account the curvature around the optimal region.
Subsequently, a second phase of cognitive validation was carried out with the adjusted PROM.
Subsequently, a second task (vtkTubeFilter) is manually added to the workflow and gets no recommendation.
Subsequently, a second generation of agents developed in recent years are currently being tested in clinical trials.
Subsequently, a second species Phytophthora kernoviae Brasier, Beales & Kirk, was also recovered from needles, though at a consistently lower rate (Dick et al. [2014]).
Subsequently, a second nitrogen pipe was buried into the goaf (the shift interval of the nitrogen injection nozzle was 50 m).
Subsequently, a second screening, which includes an MSI analysis and/or an immunohistochemical analysis is performed for the patients who match the prior clinical guidelines.
The first anodization was carried out until the residual Al film thickness approached the desired level, then the oxides were stripped away, and subsequently a second anodization was performed until the remaining Al samples were completed anodized.
Subsequently, a second set of experiments can then be conducted to further optimize the levels of the significant factors that maximize PYT production by application of a more rigorous experimental design such as the Box Behnken design (BBD) (Box et al. 1978; Khuri and Cornell 1987).
